ABSTRACT
ICAM-1 which is expressed by endothelial cells and leukocytes are observed as first markers in diseases such as transplant rejection, diabetes and atherosclerosis and in infections caused by various pathogens. In the present study, it is aimed to reveal the age-related changes in the expression of ICAM-1 on rats. Therefore, a total of 30 albino rats were taken at the age of 6, 18 and 24 months without gender discrimination. Rats were fed with standard pellet feed during the study. Afterwards, rats were sacrificed and tissue samples were collected from their rats, and the samples were evaluated under the light microscope by staining with immnunohistochemical method. It was determined that the expression of both aortic endothelial cells and endothelial cells in the media layer had been significantly increased based on the age.
ICAM-1, que se expresa en las células endoteliales y los leucocitos, se observan como primeros marcadores de enfermedades como el rechazo de trasplante, la diabetes y la aterosclerosis y en las infecciones causadas por diversos patógenos. En el presente estudio, se pretende revelar los cambios relacionados con la edad en la expresión de ICAM-1 en ratas. Un total de 30 ratas albinas fueron seleccionadas con edades de 6, 18 y 24 meses, sin discriminación de sexo. Durante el estudio se administró a las ratas alimentación estándar de pellets. Posteriormente, los animales fueron sacrificados y se recogieron muestras de sus tejidos, los cuales fueron teñidos para inmunohistoquímica y se evaluaron a través de microscopio óptico. Se determinó que la expresión tanto de células endoteliales aórticas como de células endoteliales de la capa media se incrementó significativamente en función de la edad.
Subject(s)
Animals , Rats , Aorta/cytology , Endothelium, Vascular , Intercellular Adhesion Molecule-1/metabolism , Age Factors , Rats, WistarABSTRACT
Objective To investigate the effect of nanotubes on rat aortic endothelium damage.Methods Wistar rats were intratracheally instilled with carbon nanotubes(CNTs) at the doses of 0,3.5 and 17.5 mg/kg,and with a carbon black negative control,or a quartz positive control.The rats were euthanized after 7 or 30 days of the single treatment,the oxidative stress(GSH,O_2~-?) was determined by using biochemical kits,the expression of sICAM-1 and sVCAM-1 in serum was determined by using ELISA kits and ICAM-1 and VCAM-1 on aortic endothelium was determined with immunohischemistry.Results The content of GSH and O_2~1?in serum increased with a dose-dependent and time-dependent manner.A up-regulated expression of sICAM-1 and sVCAM-1 in serum and ICAM-1 and VCAM-1 in the aortic endothelium was seen.No vascular damage was seen in the rat treated with carbon black,in those treated with high-dose CNTs obvious inflammation was revealed.Conclusion Carbon nanotubes exposure can induce oxidative stress which may be followed by the up-regulated expression of sICAM-1 and sVCAM-1 in serum and ICAM-1 and VCAM-1 in the aortic endothelium,and then the endothelium disfunction may occur.